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Risk of sudden cardiac death tied to protein overproduction in 67% of population

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Why does 67 percent of the population have a genetic mutation that leads to protein overproduction, which could lead to risk of sudden death? Quantity, not quality: Risk of sudden cardiac death tied to protein overproduction, says a new study, "An Enhancer Polymorphism at the Cardiomyocyte Intercalated Disc Protein NOS1AP Locus Is a Major Regulator of the QT Interval," reported in the June 5, 2014 issue of the The American Journal of Human Genetics. The understanding of that mechanism could lead to new drug treatment. But consumers also wonder whether any type of dietary changes could be of help to reduce the risk for those with that genetic mutation? But does the study refer to the USA or the world's population?

A genetic variant linked to sudden cardiac death leads to protein overproduction in heart cells, Johns Hopkins Medicine scientists report. Unlike many known disease-linked variants, this one lies not in a gene but in so-called noncoding DNA, a growing focus of disease research. The discovery also adds to scientific understanding of the causes of sudden cardiac death and of possible ways to prevent it, the researchers say, according to the May 27, 2014 news release, "Quantity, not quality: Risk of sudden cardiac death tied to protein overproduction."

"Traditionally, geneticists have studied gene variants that cause disease by producing an abnormal protein," says Aravinda Chakravarti, Ph.D., according to the May 27, 2014 news release, "Quantity, not quality: Risk of sudden cardiac death tied to protein overproduction." Chakravarti is a professor of medicine, pediatrics, molecular biology and genetics, and biostatistics in the McKusick-Nathans Institute of Genetic Medicine at the Johns Hopkins University School of Medicine. "We think there will turn out to be many DNA variants that, like this one, cause disease by making too much or too little of a normal protein."

Chakravarti's interest in sudden cardiac death emerged a decade ago, when it claimed several of his colleagues within a few months

An expert in complex common diseases, he and his team knew that sudden cardiac death can be caused by many conditions. They focused on one: abnormalities in what is known as cardiac repolarization — the time it takes for the heart to gear up to beat again.

The team compared the genetic sequences of tens of thousands of people with their electrocardiogram (ECG) results, identifying several regions on the genome with genetic variations associated with lengthened QT interval, a measure of cardiac repolarization, in the ECG. "The problem is that most of these variants lie outside of genes, in the noncoding DNA that controls how genes are used," Chakravarti says, according to the news release, "so it's hard to tell what genes they're affecting."

Despite the challenge, Chakravarti and his colleagues were able to home in on one suspect region of the genome housing a gene called NOS1AP. "There were many variants grouped in this area," says Ashish Kapoor, Ph.D., according to the news release Kapoor is a postdoctoral researcher in Chakravarti's laboratory, "so we cataloged all 200 that we found." The team then went through a process of elimination using genetically engineered, lab-grown cells and zebra fish to identify a variant in the noncoding DNA that affected how much protein was made by the nearby NOS1AP gene.

Next, they cultured rat heart cells and engineered them to overproduce NOS1AP. When the concentration of the protein rose in a particular type of heart cell called a cardiomyocyte, the cells' electrical properties changed in a way that is similar to the pattern seen in long QT syndrome.

Kapoor notes that 67 percent of the general population carries the NOS1AP-overproducing genetic variant

"We have observed that NOS1AP genetic variants are associated with sudden cardiac death whether or not they affect a particular person's QT interval, raising the risk by about 40 percent," he says, according to the news release. Chakravarti notes that the results also add to scientific understanding of how the heart and QT interval work — knowledge with far-reaching implications.

For example, many drugs developed for noncardiac conditions have turned out to temporarily lengthen QT interval, a side effect that only turns up after much time and money are spent on drug development. By better understanding regulation of the QT interval, researchers would be better able to predict what types of drugs could affect it.

"Hundreds of genome-wide association studies have been done to find genetic variants associated with disease, but this is one of just a handful of follow-up studies to look for the mechanism behind such a variant," Chakravarti says, according to the news release. "I think we've shown there's great value in asking why."

Other authors on the paper were Rajesh B. Sekar, Karen Fox-Talbot, Vasyl Pihur, Sumantra Chatterjee, Dan E. Arking, Marc K. Halushka and Gordon F. Tomaselli of the Johns Hopkins University School of Medicine; Nancy F. Hansen, Jim Mullikin and Eric D. Green of the National Human Genome Research Institute; Michael Morley, Jeffrey Brandimarto and Thomas P. Cappola of Perelman School of Medicine at the University of Pennsylvania; Christine S. Moravec of the Cleveland Clinic Foundation; Sara L. Pulit of the University Medical Center Utrecht; Arne Pfeufer of the Helmholtz Zentrum Munchen; Mark Ross and David Bentley of Illumina United Kingdom; Christopher Newton-Cheh of Massachusetts General Hospital; and Eric Boerwinkle of the University of Texas Health Science Center; and the QT Interval-International GWAS Consortium.

This work was supported by the National Heart, Lung and Blood Institute (grant numbers RO1 HL086694 and RO1HL105993) and the Donald W. Reynolds Foundation. Affymetrix, Inc. sells products used in the study described in this article. Aravinda Chakravarti was a paid consultant to and member of the Scientific Advisory Board of Affymetrix until December 31, 2013. This arrangement has been reviewed and approved by the Johns Hopkins University in accordance with its conflict of interest policies. Mark Ross and David Bentley are employees of Illumina, Inc., a public company that develops and markets systems for genetic analysis.

You also may be interested in news of various health research and abstracts of studies related to genes and risk factors such as, "Hopkins Scientists ID 10 Genes Associated With a Risk Factor for Sudden Cardiac Death," "New Genes Implicated in High Blood Pressure," or "Scientific Team Sequences 1092 Human Genomes To Determine Standard Range Of Human Genetic Variation."

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