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Reverse engineering your skin or an embryo: A playbook developed by researchers

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Imagine reverse engineering your skin or an embryo. Stanford University scientists have developed a 'playbook' for reverse engineering tissue. Consider the marvel of the embryo. It begins as a glob of identical cells that change shape and function as they multiply to become the cells of our lungs, muscles, nerves and all the other specialized tissues of the body.

Now, in a feat of reverse tissue engineering, Stanford University researchers have begun to unravel the complex genetic coding that allows embryonic cells to proliferate and transform into all of the specialized cells that perform myriad biological tasks.

A team of interdisciplinary researchers took lung cells from the embryos of mice, choosing samples at different points in the development cycle

Using the new technique of single-cell genomic analysis, they recorded what genes were active in each cell at each point. Though they studied lung cells, their technique is applicable to any type of cell.

"This lays out a playbook for how to do reverse tissue engineering," said Stephen Quake, PhD, according to the April 16, 2014 news release, "Stanford scientists develop 'playbook' for reverse engineering tissue." Quake is the Lee Otterson Professor in the School of Engineering and a Howard Hughes Medical Institute investigator.

The researchers' findings are described in a paper published online April 13, 2014 in the journal Nature. Quake, who also is a professor of bioengineering and of applied physics, is the senior author. The lead authors are postdoctoral scholars Barbara Treutlein, PhD, and Doug Brownfield, PhD.

The researchers used the reverse-engineering technique to study the cells in the alveoli, the small, balloon-like structures at the tips of the airways in the lungs

The alveoli serve as docking stations where blood vessels receive oxygen and deliver carbon dioxide. Treutlein and Brownfield isolated 198 lung cells from mouse embryos at three stages of gestation: 14.5 days, 16.5 days and 18.5 days (mice are usually born at 20 days). They also took some lung cells from adult mice.

They used standard enzymatic techniques to dissolve the proteins that hold the lung cells together in tissue form, then sorted out the specific alveolar cell types that were the focus of their study.

Their next steps involved newer techniques at the heart of their reverse-engineering process

Recall how eyedroppers work. Squeeze the rubber bulb to evacuate the air; plunge it into a solution to fill it with fluid; squeeze the bulb again to force the fluid out. In recent years, biotechnologists have used those basic principles to develop microfluidic devices of such precision that they can suck a single cell out of solution and isolate it in a chamber to study its genetic material.

Quake's lab has pioneered the use of microfluidic devices to study single cells. In this study, they used microfluidic devices to capture their 198 sample lung cells. Then they used single-cell genomic sequencing to measure which genes were active in each cell at each time.

How did they decode genomic activity in a single cell?

DNA in the nucleus of every cell contains the full genome for that organism. That's why it's possible to build an organism from a single cell. But only some of those genes are active in any given cell at any given time. That's why lung cells are different than hair cells; each cell has a different set of active genes directing its functions.

Genes direct cellular activity by making or "expressing" messenger RNA. Each mRNA instructs the cell to make a particular protein. Cells are essentially a group of interacting proteins. Therefore knowing which mRNAs are active offers a lens into the function of that cell at the point when it was captured in the microfluidic device.

Using this process the Stanford researchers revealed for the first time precisely which genes regulate the development of these particular lung cells at each step along the way to mature alveoli

One important finding involved the development of two key cell types at the tip of the alveoli, where the lung meets blood to perform the gas exchange that keeps us alive. Alveolar type-1 cells are the flattest cells in the body. Blood cells dock alongside them to deliver oxygen or pick up carbon dioxide. The thinness of the cell is vital to facilitating this gas transfer.

Alveolar type-2 cells are compact and cuboidal. They secrete proteins to keep the alveoli from collapsing like empty balloons, so as to maintain the inner space through which oxygen and carbon dioxide can move.

Using single-cell genomics allowed the researchers to reverse engineer the development process to show how a single progenitor cell type gives rise to both of these different, mature alveolar cells

The researchers also captured cells in transition from the progenitor to mature cell state, gaining crucial insights into the mechanism of alveolar cell differentiation. Although this study focused on lung cells, the technique — capturing individual cells at different stages of embryonic development and assessing gene activity through mRNA sequencing — can be used to reverse-engineer other tissues.

In addition to studying embryonic development, the technique could be used in clinical settings. For instance, researchers could study differences between individual cells in a tumor, improving our understanding of the stages of cancers and leading to better, more targeted therapies.

"This technology represents a quantal leap forward in our ability to apprehend the full diversity of cell types in a given population, including rare ones that could have special functions," said Tushar Desai, MD, MPH, according to the news release. Desai is an assistant professor of pulmonary and critical care medicine at Stanford and co-author of the paper. "Because a comprehensive molecular characterization of each type is achieved, including the signals they send and receive, a snapshot of the communication between individual cells will also emerge and may suggest attractive therapeutic targets in disease."

The other Stanford co-authors of the paper are Mark Krasnow, MD, PhD, professor of biochemistry and an HHMI investigator; postdoctoral scholar Angela Wu, PhD; Norma Neff, PhD, genomics core director in the Department of Bioengineering; research assistant Gary Mantalas; and F. Hernan Espinoza, PhD, an HHMI research specialist.

Supporters of the research included the National Heart Lung and Blood Institute, the National Institutes of Health, the Parker B. Francis Foundation, the Howard Hughes Medical Institute, and the Department of Bioengineering, which is jointly operated by the School of Engineering and the School of Medicine.

You also may wish to see news of another study from 2012 "Sequencing of fetal genomes using only maternal blood sample." In that study, Stanford University School of Medicine researchers have for the first time sequenced the genome of an unborn baby using only a blood sample from the mother. The new technique hinges on the fact that pregnant women have DNA from both their cells and the cells of their fetus circulating freely in their blood. Or see, "Prenatal diagnostics: Fetal genes in mother's blood."

Other studies or their abstracts are noteworthy for the curious

On another note, you may be interested in the site, "Obesity Outlook." According to the site, it’s amazing how little is known about the science underlying this condition. Why do some people seem predisposed to weight gain? And how does appetite actually work?

Devising an effective strategy to combat the obesity epidemic will require the integration of insights from neuroscience, genetics and the behavioral sciences. Or see, "Diet rapidly and reproducibly alters the human gut microbiome." Other abstracts of studies of interest the general reader might want to see include "Sperm RNA carries marks of trauma" and "Toxicology: The plastics puzzle." There also are studies or their abstracts such as "Education: Embed social awareness in science curricula " and "Alzheimer's symptoms reversed in mice." You also may want to check out, "Stress alters children's genomes." Or see, "US doctors' group says patients should have option not to know their DNA." After all, April 25, 2014 is national DNA Day. See, "2014 DNA Day | ASHG" or "National DNA Day | Facebook."

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