Jason Aldean, Blake Shelton, George Strait, Keith Urban and The Band Perry will also perform at the three-hour event, set to go live Sunday, April 6 from the MGM Grand Garden Arena in Los Vegas.
For the second year running, Bryan and Shelton will co-host the show.
During a special performance with Florida Georgia Line, Bryan, who recently scored another No. 1 hit single with the emotional "Drink A Beer," will make an appearance at the 4th Annual ACM Fan Jam at the Mandalay Bay Events Center.
Produced by Dick Clark Productions, the awards ceremony will be broadcast on the CBS Network at 8 p.m. EST. Check your local listings for more information.
Additional performers, as well as presenters, will be announced at a later date.