"She's due in June," Manning told Us Weekly on Wednesday. "We're all very excited."
Manning and his wife Abby, who met while attending college together, were married in 2008. The Manning's already have a daughter named Ava Frances, who will celebrate her second birthday on March 21.
Eli Manning's big brother and football competitor Peyton Manning and wife Ashley welcomed twins just days after Ava's birth.
“The greatest thing about being a dad is that you never realize you can love something immediately in your life,” Manning, 32, told People last June.
Congratulations on the news of Manning's wife expecting baby number two.