This exciting affair will set the table for a great day of men's and women's tennis at the Lindner Family Tennis Center in Cincinnati, Ohio.
Isner and Del Potro are scheduled to take Center Court this afternoon at 1 p.m. EST (12 p.m. CST) and tennis fans can watch all the action live on ESPN-2 and ATP's live stream feed.
The winner advances to the Sunday's round to take on either Tomas Berdych or Rafael Nadal in the 2013 Cincinnati Masters finals.
Despite coming off the biggest victory of his young career, defeating world No.1 Novak Djokovic on Friday afternoon, tennis oddsmakers still aren’t showing the unseeded American any love.
Las Vegas betting experts rely heavily on the fact that Isner, listed as a 3/2 [+150] dog, is winless in his career against Del Potro, who's a whopping 10/19 (-190) favorite to win this matchup.
All four head-to-head meetings between these giants have taken place on a hard-court surface with the seventh-ranked Argentine walking away victorious every time.
However, Isner showed much improvement in last month's Washington finals, going the distance with Del Potro (3-6, 6-1, 6-2) for the first time.