A scan revealed the injury, which was sustained in training, will keep Benteke out for at least six months.
“It’s a terrible blow for Christian and the club,” said Aston Villa manager Paul Lambert.
“He will miss the rest of the season, obviously, and also the World Cup. But he’ll work hard because that’s what he does and he’ll come back for us stronger than ever next season.”
In his first season in the Premier League, Benteke scored 28 goals in 50 appearances for club and country.
Benteke became unsettled at the end of his first season with rumors of a Tottenham switch about. His second season was a shaky one, with his goal return down to 11 in 29 appearances.
With Benteke out this summer, Belgium will likely turn to Romelu Lukaku to lead the line up front.
Meanwhile, Aston Villa will fight to stay in the Premier League currently sitting in 12th and eight points clear of the relegation zone.