The child - Denise Hernandez - was last seen by her family in their residence on Wells Street late Sunday evening when they put her to bed.
Immokalee is a small agricultural town about 100 miles northwest of Fort Lauderdale.
Hernandez was discovered missing around 11:30 p.m. Sunday.
According to Florida Department of Law Enforcement, Hernandez is described as 3 feet tall, 40 pounds with black hair and brown eyes. A picture of the child was not immediately available.
She was wearing a white nightgown with multi-colored stripes.
Anyone with information about the whereabouts of two-year old Denise Hernandez should immediately call 911, contact the nearest law enforcement agency or call the Collier County Sheriff's Office at 239-252-9300.
This report is accurate as of 5:40 a.m. Monday morning.